hardware assembly to perform transcranial photobiomodulation for brain drainage in aged mice

Enhancing Brain Drainage During Sleep with Transcranial Photobiomodulation(경두개 광생물조절을 통한 수면 중 뇌 배출 향상)

Meningeal lymphatic vessels (MLVs) play an important role in the removal of toxins and metabolites from brain tissues1,2,3. Damage of MLVs in various brain diseases, including tumors, traumatic brain injuries, hemorrhages, and neurodegenerative processes, is accompanied by a decrease in the MLV functions leading to the progression of these pathologies1,2,3,4,5,6. Therefore, the development of methods for the stimulation of MLVs opens new horizons in the emergence of effective technologies for the treatment of brain diseases. Recently, non-invasive technology for effective transcranial photobiomodulation (tPBM) has been proposed to stimulate MLVs and remove toxins such as blood and A&#946; from the brain5,7,8,9,10,11,12. It is interesting to note that deep sleep is a natural factor for the activation of lymphatic drainage processes in the brain13,14. Based on this fact, it is logical to assume that the tPBM of MLVs during sleep may have more effective therapeutic effects than during wakefulness9,11,12,15. However, there are currently no commercial technologies for tPBM during sleep16. In addition, animal experiments to study the therapeutic effects of tPBM are performed under anesthesia, which is required to accurately deliver light to the brain. However, anesthesia significantly affects the brain&#39;s drainage, which reduces the quality of research results17.
A&#946; is a metabolic product of normal neural activity18. As it was established in cultured rat cortical neurons, A&#946; is released from them at high rates into the extracellular space (2-4 molecules/neuron/s for A&#946;)19. There is evidence that the dissolved form of A&#946;, located in the extracellular and perivascular spaces, is most toxic to neurons and synapses20. The soluble A&#946; is rapidly cleared from the human brain during 1-2.5 h21. MLVs are the tunnels for removal of the soluble A&#946; from the brain1,7 that declines with age, leading to the accumulation of A&#946; in the aged brain1,22. There is evidence that extracellular abnormalities of A&#946; levels in the brain correlate with cognitive performance in aging and are associated with the development of Alzheimer&#39;s disease (AD)23,24. Therefore, aged and old rodents are considered alternatives to transgenic models for the study of amyloidosis, including AD25,26.
This study presents an original and portable tPBM technology under electroencephalographic (EEG) control of deep or non-rapid eye movement (NREM) sleep in non-anesthetized male C57BL/6 mice of different ages to stimulate lymphatic clearance of A&#946; from the brain into the peripheral lymphatic system (the deep cervical lymph nodes, dcLNs).

저자 스포트라이트: 수면 관련 뇌 질환의 독소 제거를 위한 광자극 기술 개발

생쥐 뇌에서 독소의 림프 제거 자극을 위한 수면의 뇌파 제어 하에서의 광생물 조절

Aged Mice의 뇌 배수를 위한 경두개 광생물 조절을 수행하기 위한 하드웨어 조립

hardware-assembly-to-perform-transcranial-photobiomodulation-for

Research

JoVE Journal

Neuroscience

Hardware Assembly to Perform Transcranial Photobiomodulation for Brain Drainage in Aged Mice

36 조회수. 하드웨어 조립을 시작하려면 발광 다이오드 또는 LED를 인쇄 회로 기판 또는 PCB에 납땜하고 LED와 자석을 3D 프레임에 놓습니다. 그런 다음 LED 양극과 Arduino의 5볼트 포트 사이에 저항을 연결합니다. 다음으로, LED 음극을 금속 산화물 반도체 전계 효과 트랜지스터 또는 MOSFET 드레인에 연결하고 MOSFET 소스를 접지에 고정합니다.MOSFET 게이트와 접지 ?...

비디오: Aged Mice의 뇌 배수를 위한 경두개 광생물 조절을 수행하기 위한 하드웨어 조립

Photobiomodulation Under Electroencephalographic Controls of Sleep for Stimulation of Lymphatic Removal of Toxins from Mouse Brain

The meningeal lymphatic vessels (MLVs) play an important role in the removal of toxins from the brain. The development of innovative technologies for the stimulation of MLV functions is a promising direction in the progress of the treatment of various brain diseases associated with MLV abnormalities, including Alzheimer&#39;s and Parkinson&#39;s diseases, brain tumors, traumatic brain injuries, and intracranial hemorrhages. Sleep is a natural state when the brain&#39;s drainage processes are most active. Therefore, stimulation of the brain&#39;s drainage and MLVs during sleep may have the most pronounced therapeutic effects. However, such commercial technologies do not currently exist.
This study presents a new portable technology of transcranial photobiomodulation (tPBM) under electroencephalographic (EEG) control of sleep designed to photo-stimulate removal of toxins (e.g., soluble amyloid beta (A&#946;)) from the brain of aged BALB/c mice with the ability to compare the therapeutic effectiveness of different optical resources. The technology can be used in the natural condition of a home cage without anesthesia, maintaining the motor activity of mice. These data open up new prospects for developing non-invasive and clinically promising photo-technologies for the correction of age-related changes in the MLV functions and brain&#39;s drainage processes and for effectively cleansing brain tissues from metabolites and toxins. This technology is intended both for preclinical studies of the functions of the sleeping brain and for developing clinically relevant treatments for sleep-related brain diseases.

This study presents an original and portable tPBM technology under electroencephalographic (EEG) control of deep or non-rapid eye movement (NREM) sleep in non-anesthetized male C57BL/6 mice of different ages to stimulate lymphatic clearance of A&#946; from the brain into the peripheral lymphatic system (the deep cervical lymph nodes, dcLNs).

The meningeal lymphatic vessels (MLVs) play an important role in the removal of toxins from the brain. The development of innovative technologies for the ...