PCR Verification of Transgenic Parasite with Desired Modification of the cdpk1 Locus for Elucidating Plasmodium falciparum Kinase Resistance Mechanisms

To begin, treat the transformed Plasmodium falciparum parasites with the desired drugs. Following two to three days of parasite growth, centrifuge 500 microliters of culture in a micro centrifuge tube at 2, 400 G for five minutes. To lyse the RBCs