HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

0:59

Isolation of Leukemic Lymphocytes from Blood Samples of CCL Patients

2:09

Purification of Leukemic B Cells by Negative Isolation

3:27

Program the HPLC Method

5:07

Pretreatment with the Inhibitors and Incubation with the Substrate (AMP)

6:16

Samples Preparation for HPLC and HPLC Measurements of Purines

8:14

Results: Quantification of Purine Compounds in Freshly Purified CLL Cells from a Representative CD73⁺ Patient

9:36

Conclusion