December 12th, 2016
•Here, the experimental protocols are described for preparing Drosophila at different developmental stages and performing longitudinal optical imaging of Drosophila heartbeats using a custom optical coherence microscopy (OCM) system. The cardiac morphological and dynamical changes can be quantitatively characterized by analyzing the heart structural and functional parameters from OCM images.
Related Videos
Proper Care and Cleaning of the Microscope
Phase Contrast and Differential Interference Contrast (DIC) Microscopy
Born Normalization for Fluorescence Optical Projection Tomography for Whole Heart Imaging
Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies
Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
Preparation of Drosophila S2 cells for Light Microscopy
Confocal Imaging of Single Mitochondrial Superoxide Flashes in Intact Heart or In Vivo
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
Copyright © 2024 MyJoVE Corporation. 판권 소유