Treatment of HeLa Cells with Biotinylated Psoralen RNA Extraction, and Fragmentation
2:25
RNA Size Selection and Elution
4:41
Enrichment of RNA Crosslinking Regions
5:57
Proximity Ligation
7:34
Reverse Crosslinking of Biotinylated Psoralen, and Amplification of cDNA
9:13
The SPLASH Workflow and Representative Results
10:50
Conclusion
필기록
The overall goal of this video is to describe the method for sequencing of psoralen crosslinked ligated and selected hybrids or SPLASH in which pair-wise in vivo RNA-RNA interactions are mapped in a genome-wide manner. This technique is a simple,
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Here, we detail the method of Sequencing of Psoralen crosslinked, Ligated, and Selected Hybrids (SPLASH), which enables genome-wide mapping of intramolecular and intermolecular RNA-RNA interactions in vivo. SPLASH can be applied to study RNA interactomes of organisms including yeast, bacteria and humans.