Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance

0:41

Protocol

1:06

Isolation: Soil Dilution Method

2:29

Isolation from Insect Cadavers

2:55

Isolation: Bait Method

3:44

Identification of Entomopathogenic Fungi

4:11

Representative Results: Entomopathogenic Fungi

4:34

Insect Bioassay of Metarhizium Isolates

6:23

Representative Results: Metarhizium Isolates

6:59

Production of Spores: Development of Mycelial Inoculum

7:33

Production of Spores: Solid State Fermentation for Production of Spores

9:22

Production of Spores: Precentatge Germination Studies

9:55

Production of Spores: Spore Sedimentation Rate

10:32

Representative Results: Production of Spores

10:41

Field Performance of Metarhizium Isolate M34412: Aqueous and Oil Based Spore Formulations

11:59

Representative Results: Effect of Metarhizium anisopliae on Non-target Anthropods

12:29

Quality Control Parameteres

13:37

Discussion and Conclusion