Endothelial Cell Culture and Tissue Culture Conditions
2:42
Evaluate Endothelial Factor Released on Leukocyte Activation by Adhesion Assays
4:12
Leukocyte Adhesion Assay
7:42
Test Endothelial Activation by Leukocyte Endothelium Co-adhesion Assay
9:59
Results: Activation Parameters of LPS-stimulated Endothelial Cells and Influence on Leukocyte Behavior
11:52
Conclusion
필기록
The overall goal of this screening assay is to characterize novel endothelial regulators involved in the inflammatory response of interest for designing new therapeutic strategies against inflammatory disorders.This method can help answer key ques
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Vascular endothelium tightly controls leukocyte recruitment. Inadequate leukocyte extravasation contributes to human inflammatory diseases. Therefore, searching for novel regulatory elements of endothelial activation is necessary to design improved therapies for inflammatory disorders. Here, we describe a comprehensive methodology to characterize novel endothelial regulators that can modify leukocyte trafficking during inflammation.