The overall goal of this procedure is to analyze DNA polymerase activity on a single nucleotide level. This method can help answer key questions in the DNA polymerase field such as which specific steps of DNA synthesis are limited by DNA modificat
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This protocol describes the characterization of DNA polymerase synthesis of modified DNA through observation of changes to near-infrared fluorescently labeled DNA using gel electrophoresis and gel imaging. Acrylamide gels are used for high resolution imaging of the separation of short nucleic acids, which migrate at different rates depending on size.