Injection Efficiency and Transgene Effects Assessment
6:06
Results: Hydrodynamic Renal Pelvis Injection Results in Kidney-specific Gene Expression
7:25
Conclusion
필기록
The overall goal of this surgical procedure is to produce protein expression from plasmid DNA in the adult mouse kidney. This method is a new tool to address key questions in nephrology, such as, how the expression of a protein of interest affects
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This protocol describes a method to inject plasmid DNA into the mouse kidney via the renal pelvis to produce transgene expression specifically in the kidney.