Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis

1:40

Construct the pET-SUMO-EGFP Plasmid and Induce EGFP-expression

4:28

Mouse Peritoneal Macrophage Isolation and Primary Culture

6:57

Macrophage Phagocytosis Assay using Flourescence Microscopy

8:28

Macrophage Phagocytosis Assay using Flow Cytometer

11:05

Results

11:45

Conclusion