Microinjecting DNA/DOTAP into Eyebuds of One Day Old Xenopus Embryos
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Results: Control and Mutant Optic Axonal Arbors
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Conclusion
필기록
In this video, we demonstrate how to express exogenous DNA constructs in optic neurons and how to image individual GFP-expressing optic axonal arbors in intact, living Xenopus laevis tadpoles. This is an inexpensive, simple procedure for transient
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This protocol aims to demonstrate how to microinject a DNA/DOTAP mixture into eyebuds of one day old Xenopus laevis embryos, and how to image and reconstruct individual green fluorescent protein (GFP) expressing optic axonal arbors in tectal midbrains of intact, living Xenopus tadpoles.