Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

0:55

Embryo Staining and Embedding

3:03

Methanol Dehydration and Tissue Clearing

4:04

Embryo Mounting

4:40

Third Pharyngeal Arch (PA) Surfacing

5:57

Surfaced Structure Masking

7:01

Endothelial Cell Quantification

8:19

Results: Representative PA and PAA Surfacing and EC Quantification

9:28

Conclusion