Results: Representative Image Analysis and Fluorescence Readout
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Conclusion
필기록
Live-Cell Imaging of Single-Cell Arrays called LISCA are also an automated readout of the fluorescent time courses of hundreds of individual cells. The advantage of the approach is that individual cell responses are recorded from spatially isolate
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We present a method for the acquisition of fluorescence reporter time courses from single cells using micropatterned arrays. The protocol describes the preparation of single-cell arrays, the setup and operation of live-cell scanning time-lapse microscopy and an open-source image analysis tool for automated preselection, visual control and tracking of cell-integrated fluorescence time courses per adhesion site.