Stimulator or Inhibitor Titration Using the Two-Plate Assay Protocol with Adherent Cells
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Results: Two-Plate and One-Plate Detection of STAT Proteins in Adherent and Suspension Cell Lines, and Intraplate Variability Study
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Conclusion
필기록
The TR-FRET based assays provide new cost effective tools for the screening and pharmacological characterization of specific and selective modulators of the JAK/STAT signaling pathways. This technique is much easier, rapid, reproducible, and robus
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Time-resolved Förster resonance energy transfer cell-based assay protocols are described for the simple, specific, sensitive, and robust quantification of endogenous phosphorylated signal transducer and activator of transcription (STAT) 1/3/4/5/6 proteins in cell lysates in a 384-well format.