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Federal University of Minas Gerais

2 ARTICLES PUBLISHED IN JoVE

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Biology

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
Rafael M. Rezende *1, Mateus E. Lopes *2, Gustavo B Menezes 2, Howard L. Weiner 1
1Ann Romney Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, 2Center for Gastrointestinal Biology, Federal University of Minas Gerais

This protocol describes a technique to image different cell populations in draining lymph nodes without alterations in the organ structure.

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Immunology and Infection

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice
Ricardo Gonçalves 1, Gabriela Kaliff Teófilo Murta 1, Izabela Aparecida de Souza 1, David M. Mosser 2
1Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, 2Department of Cell Biology and Molecular Genetics, University of Maryland

The present protocol describes the isolation and culture of bone marrow-derived macrophages from mice.

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