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Understanding Concentration and Measuring Volumes

Overzicht

Solutions are utilized to some degree in almost all biological research applications. Therefore understanding how to measure and manipulate them is imperative to any experiment. In this video, concepts in preparing solutions are introduced. Solutions consist of a solute dissolved in solvent to yield a homogeneous mixture of molecular substances. Solutions are generally identified by their components and corresponding concentrations. Concentrated solutions are diluted through various methods, such as serial dilution. This video also lays a foundation for the accurate preparation of solutions. For example, the video reviews how to measure volumes with precision through use of the appropriate volumetric container as well as how to read the volume when a meniscus is present. Some applications for measuring volumes are then presented. Gel electrophoresis is a commonly used laboratory procedure which requires preparation of a percent weight volume solution as well as parallel dilution of a concentrated stock solution. Use of a serial dilution to prepare standards for generating a standard curve in protein quantitation is also demonstrated.

Procedure

Understanding the concepts behind solution concentration and measuring volumes in the lab are two important aspects of nearly every experiment.

Solutions are made up of a solute dissolved in solvent to yield a homogeneous mixture.

Solutions are generally identified by their components and corresponding concentrations.

To correctly arrive at the correct solution concentration, you must be familiar with the many different containers available for volume measurements.

Poor technique when measur

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Tags

Solution ConcentrationMeasuring VolumesSoluteSolventHomogeneous MixtureComponentsConcentrationsSolution ConcentrationMolarityMol Of SoluteLiter Of SolutionPercent ConcentrationsWeight Of SoluteUnit Volume Of SolventPercent Weight volume SolutionLiquid SolutePercent Volume volume SolutionStock Solutions

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0:00

Overview

1:02

Quantifying and Diluting Solutions

3:43

Measuring Volumes

6:34

Applications

8:10

Summary

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