Taking Advantage of Reduced Droplet-surface Interaction to Optimize Transport of Bioanalytes in Digital Microfluidics

Podsumowanie

The protocol for fabrication and operation of field dewetting devices (Field-DW) is described, as well as the preliminary studies of the effects of electric fields on droplet contents.

Streszczenie

Digital microfluidics (DMF), a technique for manipulation of droplets, is a promising alternative for the development of “lab-on-a-chip” platforms. Often, droplet motion relies on the wetting of a surface, directly associated with the application of an electric field; surface interactions, however, make motion dependent on droplet contents, limiting the breadth of applications of the technique.

Some alternatives have been presented to minimize this dependence. However, they rely on the addition of extra chemical species to the droplet or its surroundings, which could potentially interact with droplet moieties. Addressing this challenge, our group recently developed Field-DW devices to allow the transport of cells and proteins in DMF, without extra additives.

Here, the protocol for device fabrication and operation is provided, including the electronic interface for motion control. We also continue the studies with the devices, showing that multicellular, relatively large, model organisms can also be transported, arguably unaffected by the electric fields required for device operation.

Wprowadzenie

The miniaturization of devices that work with liquids is of paramount importance for the development of “lab-on-a-chip” platforms. In this direction, the last two decades have witnessed a significant progress in the field of microfluidics, with a variety of applications.^1-5 Contrasting with the transport of fluid in enclosed channels (channel microfluidics), DMF manipulates droplets on arrays of electrodes. One of the most attractive merits of this technique is the absence of movable parts to transport fluids, and motion is instantly stopped by turning off electric signals.

However, droplet motion is dependent on droplet contents, certainly an undesirable characteristic for a universal “lab-on-a-chip” platform. Droplets containing proteins and other analytes stick to device surfaces, becoming unmovable. Arguably, this has been the major limitation for broadening the scope of DMF applications;^6-8 alternatives to minimize the unwanted surface fouling involve the addition of extra chemical species to the droplet or its surroundings, which could potentially affect droplet content.

Previously, our group developed a device to allow the transport of cells and proteins in DMF, without extra additives (Field-DW devices).⁹ This was achieved by combining a surface based on candle soot,¹⁰ with a device geometry that favors droplet rolling and leads to an upward force on the droplet, further decreasing droplet-surface interaction. In this approach, droplet motion is not associated with surface wetting.¹¹

The goal of the detailed method described below is to produce a DMF device capable of transporting droplets containing proteins, cells, and entire organisms, without extra additives. The Field-DW devices pave the way for fully controlled platforms working largely independently of droplet chemistry.

Here, we also present simulations showing that, despite the high voltage required for device operation, the voltage drop across the droplet is a small fraction of the applied voltage, indicating negligible effects on bioanalytes inside the droplet. In fact, preliminary tests with Caenorhabditis elegans (C. elegans), a nematode used for a variety of studies in biology, show that worms swim undisturbed as voltages are applied.

Protokół

NOTE: In the procedures described below, laboratory safety guidelines must always be followed. Of particular importance is the safety when dealing with high voltage (> 500 V) and handling chemicals.

1. Coating of a Conductive Substrate with Candle Soot

1. Cut copper metal into rectangles (75 x 43 mm, 0.5 mm thick). Clean each copper substrate by immersion in copper etchant for about 30 sec, wash with tap water for about 20 sec, and dry with paper.
   NOTE: If using Method 1 below, change the dimensions to 75 x 25 mm to fit into the machine.
2. Sweep a lit paraffin candle under the copper substrate for 30–45 sec, to obtain an approximately uniform soot coating (about 40 µm thick). Keep the substrate at ~1 cm inside the flame. Do not touch the fragile soot surface.

2. Protecting the Soot Layer with Coating

NOTE: The soot layer is very fragile, and must be coated for protection. Two simple alternatives (methods 1 and 2 below) are suggested here, but more robust protocols are currently under development.

1. Method 1
   1. Load the sample into the metal evaporator or sputtering system. Following the operation procedures of the system, evacuate the chamber, and start controlled deposition of gold onto the soot layer (150–200 nm). Let the device cool down to room temperature.
   2. Dip-coat the metalized substrate in a 1-dodecanethiol solution (1% v/v, in 95% ethanol, ACS/USP grade), for 10 min inside a chemical hood. Then, holding the device at an angle close to 60°, gently wash the surface with several droplets of ethanol only. Let the devices dry, overnight.
2. Method 2
   1. In a chemical hood, immediately after coating the substrate with soot and while the substrate is still warm from the candle flame, deposit some droplets of fluorinated liquid on one side of the substrate, and tilt the substrate to an angle close to 90°. Deposit more droplets, and let them roll over the entire soot surface.
      NOTE: When the droplet falls on a spot, soot will be washed away from that area. Let the droplets of fluorinated liquid spread as much as possible.
   2. Bake the substrate on a hot plate (160 °C for 15 min) inside a chemical hood.
   3. Let the substrate sit overnight at room temperature before usage. Store indefinitely.

3. Fabrication of Top Electrodes (Adapted from Abdelgawad et al.¹²)

1. Draw the electrodes using graphic design software. Each electrode is 2 mm long, 0.3 mm wide, and the gap between electrodes is 0.3 mm. The gap between contacts (to snap into the connector, see below) is 2.3 mm (Figure 1).
2. Trim a flexible copper laminate (35 µm thick) into the Monarch format (3.87 x 7.5 inches). Use other sizes if compatible with the printer. Load the laminate into the manual feed tray of a color printer.
3. Make sure to use “rich black”, or “registration black”, when printing on the copper sheet (see Abdelgawad et al.¹² for details) to allow a denser layer of black ink on the copper substrate, protecting the printed pattern during etching. Let the printed substrate dry completely, overnight.
4. Inside a chemical hood, warm up (40 °C) a beaker with 50 ml of copper etchant. Dip the printed laminate in the beaker, and gently shake it in the solution for about 10 min. Etching time depends on the copper etchant solution. Every few minutes, check the corrosion and see if the pattern is intact.
5. Carefully wash the laminate with water, and remove the coating with acetone and ethanol in the chemical hood. Wash once again, and gently dry the laminate with paper towel.
6. Carefully attach the laminate with electrodes to a glass slide (75 x 25 mm, ~1 mm thick), using double sided tape. Avoid air pockets.
7. Attach a film of perfluoroalkoxy PFA to the electrodes using tape. This serves to prevent accidental contact of the electrodes with the droplet, which damages top electrodes due to short-circuit.

4. The Electronic Interface (Circuit in Figure 2)

1. Solder the relays and the capacitors C to a universal circuit board.
2. Assemble the remainder of the 10 relay drivers on a solderless breadboard for electronic circuits.
3. Wire each relay driver’s input to a channel in the control board.
4. Carefully snap the top electrodes into a connector (Figure 3). Wire each relay driver’s output to a top electrode, as shown in the figure. Note that there is a grounded connector contact between a pair of wires from relays, to minimize electrical noise.
   NOTE: The connector sits on an adjustable platform to control the distance (0.1–0.5 mm) between top and bottom (soot-coated) substrate.
5. Use a program to control timing for high voltage (HV) application (about 0.8 sec) to 4 electrodes at the same time, shifting 1 electrode in the direction of motion (i.e., for 0.8 sec, actuate 1234; then 2345, 3456, etc., 0.8 sec for each group, and then backwards, so droplet moves in the opposite direction as well).

5. Droplet Visualization and Handling

1. To record droplet motion, use the visualization system, which is composed of a 24X – 96X magnification assembly combined with a CCD camera. Connect the video recorder to the camera using S-video.
2. Pipette a 4 µl droplet containing C. elegans in media on the bottom of the soot-coated substrate.
3. Bring the top electrodes to ~0.3 mm above the droplet. The droplet should be close to the middle, just below the fifth electrode, for easier operation.
4. Turn on the electronic interface and high voltage (500 V_RMS), and adjust the top electrode distance to the droplet until it starts moving. Do not let the top electrodes touch the droplet.
5. Collect data by recording the number of successful droplet transfers on the device in response to electric pulses. A successful experiment is characterized by at least 700 droplet transfers, i.e., one transfer after each electric pulse.
6. Collect data continuously, until the droplet does not move anymore in response to 5 to 10 pulses.
   NOTE: When the surface starts to degrade, motion could be restored by bringing the top electrodes closer to the droplet.

Wyniki

Previously, we have used Field-DW devices to allow the motion of proteins in DMF. In particular, droplets with bovine serum albumin (BSA) could be moved at a concentration 2,000 times higher than previously reported by other authors (without additives). This was due to the reduced interaction between droplet and surface; Figure 4 shows a droplet containing fluorescently-tagged BSA (see Freire et al.⁹ for more information on the experiments). The first picture on the left shows the dro...

Dyskusje

The most critical step of the protocol is the protection of the soot layer, directly associated with the success in moving droplets. Metallizing the soot layer (method 1 above) allows close to 100% of fabrication success. However, the maximum operation time is about 10 min; possibly, droplet fractions are wetting the soot through holes in the metal layer. Coating the soot layer with the fluorinated liquid is the easiest and fastest alternative, and requires minimum resources, but only 40–50% of the fabricated subst...

Materiały

Name	Company	Catalog Number	Comments
Paraffin candle			Any paraffin candle
Sputtering system	Denton Vacuum, Moorestown, NJ		Sputter coater Desk V HP equipped with an Au target.
1-dodecanethiol	Sigma-Aldrich	471364
Teflon	Dupont	AF-1600
Fluorinert FC-40	Sigma-Aldrich	F9755	Fluorinated liquid: Prepare Teflon-AF resin in Fluorinert FC-40, 1:100 (w/w), to create the hydrophobic coating.
Graphic design software -Adobe Illustrator	Adobe Systems		Other softwares might be used as well.
Copper laminate	Dupont	LF9110
Laser Printer	Xerox	Phaser 6360 or similar	Check for the compatibility with "rich black" or "registration black" (see text).
Copper Etchant	Transene	CE-100
Perfluoroalkoxy (PFA) film	McMaster-Carr	84955K22
Breadboard	Allied Electronics	70012450 or similar	Large enough to allow the assemble of 10 drivers.
Universal circuit board	Allied Electronics	70219535 or similar
Connector	Allied Electronics	5145154-8 or similar
Control board and control program (LabView software)	National Instruments	NI-6229 or similar
High-voltage amplifier	Trek	PZD700
Capacitors C and C1, 100 nF, 60 V	Allied	8817183
Transistor T, NPN	Allied	9350289
Diode D, 1N4007	Allied	2660007
Relay	Allied	8862527
Visualization system	Edmund Optics	VZM 200i or similar	System magnification 24X – 96X. It is combined with a Hitachi KP-D20B 1/2 in CCD Color Camera.
Recorder	Sony	GV-D1000 NTSC or similar	It is connected to the camera by an S-video cable.
Simulations	COMSOL Multiphysics	V. 4.4