Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach

Podsumowanie

The Claisen-Schmidt condensation reaction is an important methodology for the generation of methine-bridged conjugated bicyclic aromatic compounds. Through utilizing a base-mediated variant of the aldol reaction, a range of fluorescent and/or biologically relevant molecules can be accessed through a generally inexpensive and operationally simple synthetic approach.

Streszczenie

Methine-bridged conjugated bicyclic aromatic compounds are common constituents of a range of biologically relevant molecules such as porphyrins, dipyrrinones, and pharmaceuticals. Additionally, restricted rotation of these systems often results in highly to moderately fluorescent systems as observed in 3H,5H-dipyrrolo[1,2-c:2',1'-f]pyrimidin-3-ones, xanthoglows, pyrroloindolizinedione analogs, BODIPY analogs, and the phenolic and imidazolinone ring systems of Green Fluorescent Protein (GFP). This manuscript describes an inexpensive and operationally simple method of performing a Claisen-Schmidt condensation to generate a series of fluorescent pH dependent pyrazole/imidazole/isoindolone dipyrrinone analogs. While the methodology illustrates the synthesis of dipyrrinone analogs, it can be translated to produce a wide range of conjugated bicyclic aromatic compounds. The Claisen-Schmidt condensation reaction utilized in this method is limited in scope to nucleophiles and electrophiles that are enolizable under basic conditions (nucleophile component) and non-enolizable aldehydes (electrophile component). Additionally, both the nucleophilic and electrophilic reactants must contain functional groups that will not inadvertently react with hydroxide. Despite these limitations, this methodology offers access to completely novel systems that can be employed as biological or molecular probes.

Wprowadzenie

A number of conjugated bicyclic systems, in which two aromatic rings are linked by a monomethine bridge, undergo isomerization via bond rotation, when excited with a photon (Figure 1A)^1,2,3,4,5. The excited isomer will generally relax to the ground state through non-radiative decay processes⁶. If the energy barrier to bond rotation is increased to a large enough extent, it is possible to restrict or prevent the photoisomerization. Instead, photonic excitation results in an excited singlet state that often relaxes via fluorescence rather than non-radiative decay (Figure 1B). Restraining photoisomerization is most commonly accomplished by mechanically restricting bond rotation through tethering the two aromatic ring systems by covalent linkages, thereby locking the molecule into a particular isomeric state. This approach has been utilized to create several different fluorescent tricyclic dipyrrinone and dipyrrolemethane analogs such as: 3H,5H-dipyrrolo[1,2-c:2',1'-f]pyrimidin-3-ones (1), xanthoglows (2)^6,7, pyrroloindolizinedione analogs (3)⁸, and BODIPY analogs⁹ (4, Figure 2) whereby the pyrrolidine and/or pyrrole ring systems are tethered with methylene, carbonyl, or boron difluoro linkers. Typically, 1-4 possess Φ_F > 0.7 suggesting these systems are very efficient as fluorophore units.

It is also possible to restrict photoisomerization through means other than covalently linking the ring systems. For example, the phenolic and imidazolinone rings (Figure 2) of Green Fluorescent Protein (GFP) are restricted to rotation by the protein environment; the restrictive setting increases the quantum yield by three orders of magnitude in comparison to the same chromophore unit in free solution¹⁰. It is believed that the protein scaffold of GFP provides a rotational barrier through steric and electrostatic effects¹¹. Recently, our group in collaboration with the Odoh group at the University of Nevada, Reno discovered another fluorophore system that bears structural similarity to the dipyrrinone-based xanthoglow systems (Figure 2)¹². These dipyrrinone analogs, however, differ from the xanthoglow system in that intramolecular hydrogen bonds, rather than covalent bonds, deter photoisomerization and result in a fluorescent bicyclic system. Furthermore, the pyrazole, imidazole, and isoindolone dipyrrinone analogs can hydrogen bond in protonated and deprotonated states; deprotonation results in the red-shifting of both the excitation and emission wavelengths, likely due to a change in the electronic nature of the system. While hydrogen bonding has been reported to increase quantum yields though restricted rotation^13,14,15,16, we are unaware of any other fluorophore system in which restricted isomerization serves as a mode of fluorescence in both protonated and deprotonated states of the molecule. Therefore, these pH dependent dipyrrinone fluorophores are unique in that respect.

In this video, we focus on the synthesis and chemical characterization of the fluorescent dipyrrinone analog series. In particular, there is an emphasis placed on the Claisen-Schmidt condensation methodology that was used to construct the complete series of fluorescent analogs. This reaction relies on the generation of a base-mediated vinylogous enolate ion which attacks an aldehyde group, to produce an alcohol that subsequently undergoes elimination. For the dipyrrinone analog series, a pyrrolinone/isoindolone is converted to an enolate to facilitate an attack upon an aldehyde group attached to a pyrazole or imidazole ring (Figure 3); after elimination a fully conjugated bicyclic system, linked by a methine-bridge, is formed. It is noteworthy that the entire series of dipyrrinone analogs can be constructed from readily available commercial materials and can be produced in a single one-pot reaction sequence typically in moderate to high yields (yields range from approximately 50-95%). Since most of the dipyrrinone analogs are highly crystalline in nature, very little purification outside of standard workup conditions is required to produce analytically pure samples. Consequently, this fluorophore system requires only a few steps to access from readily available commercial materials and can be synthesized, purified, and prepared for analytical or biological studies in a relatively short time frame.

Protokół

1. General Procedure for Synthesis of Dipyrrinone Analogs 16-25

1. Dissolve pyrrolinone/isoindolone (1.00 mmol) and the corresponding pyrazole/imidazole aldehyde (1.00 mmol) in 5.0 mL of ethanol in a round-bottom flask.
2. Add aqueous KOH (24.0 mmol, 10 M, 2.40 mL) to the flask in one portion.
3. Stir and reflux the mixture until reaction completion is confirmed by TLC (See Table 1 for a list of reaction times). A TLC eluent of 10% methanol in dichloromethane was used and the analogs have been observed to possess R_f values in or around the range of 0.62 to 0.86. Apply a sufficient amount of vacuum grease or use a PTFE sleeve at the interphase of glass joints to prevent the glass of the condenser and the round-bottom flask from seizing under high temperatures and basic conditions.
4. Allow the reaction mixture to cool to room temperature and evaporate volatiles under reduced pressure using a rotary evaporator.
5. Cool the reaction mixture to 0 °C using an ice bath.
6. Neutralize the remaining oily mixture by adding acetic acid (30.0 mmol, 1.70 mL) in one portion.
7. Purify the resulting product material using vacuum filtration (see Step 2a, for compounds: 17, 18, 20-22, 24 and 25) or use liquid-liquid extraction/column chromatography (see Step 2b, for compounds: 16, 19, and 23).

2. Procedure Purification

1. via Vacuum Filtration
   1. Fit a Hirsch funnel to a side-arm flask using a fitted rubber adapter.
   2. Apply a piece of round filter paper to the Hirsch funnel and lightly wet using deionized water to allow adherence to the funnel.
   3. Connect a vacuum source to the side-arm of the flask and ensure that there is a sufficient vacuum seal by making sure none of the glassware can be pulled apart while under vacuum.
   4. Pour the flask containing the crystallized product over the vacuum filter and allow to filter. Rinse the crystals with 10 mL of ice-cold deionized water.
   5. Allow the filtered crystals to continue to dry on top of the filter paper, while still connected to the vacuum source, following the filtration of all liquids.
   6. Collect the filtered crystals and place in a 25 mL round-bottom flask.
   7. Attach the round-bottom flask to a fritted ground glass joint/vacuum line adapter. Secure the glass joint union with a keck clip.
   8. To the ribbed end of the glass high vacuum line adapter, attach a vacuum line that is routed to a high vacuum pump and adequately cool a vacuum trap (using coolant such as liquid nitrogen or dry ice/acetone) to condense any volatile materials that may evaporate from the crystalline material. Turn on the high vacuum pump to ensure complete evaporation of any trace amounts of remaining solvent from the crystals.
   9. Allow the crystals to dry under high vacuum for a minimum of 1 h. Remove the round-bottom flask from the vacuum line/adapter, turn off the high vacuum pump, and clean out the vacuum trap.
2. Procedure Purification via Column Chromatography
   1. Dilute the acetic acid treated reaction mixture contents (from step 1.6) with 10 mL of deionized water and transfer into a separatory funnel. Add 10 mL of dichloromethane to the separatory funnel. Gently shake and vent the separatory funnel in order to separate the two layers.
   2. Extract the aqueous layer using subsequent portions of dichloromethane (3 x 5 mL). Combine the organic fractions and dry using anhydrous Na₂SO₄. Decant and remove all volatiles under reduced pressure using a rotary evaporator.
   3. Dilute the residue obtained from step 2.2.2. with 5 mL of CH₂Cl₂. Perform flash column chromatography using approximately 75 g of silica gel. Elute the sample with a solution of 10% methanol in dichloromethane.
   4. Remove the collected fractions of solvent under reduced pressure using a rotary evaporator. Transfer the solid residue to a 25 mL round-bottom flask using approximately 10 mL of CH₂Cl₂. Remove the solvent under reduced pressure using a rotary evaporator.
   5. Dry the remaining solid residue under high vacuum as previously described in steps 2.1.7 - 2.1.9.

3. Molar Absorptivity Acquisition and UV/Vis pK_a Studies for Analogs 16-25

1. Create compound stock solutions for UV/Vis Spectrophotometry for analogs 16 - 25.
   1. Weigh out 10 μmol of the selected dipyrrinone analog (16 - 25) and add it to a 10 mL volumetric flask.
   2. Add DMSO to the 10.0 mL mark on the volumetric flask.
      ​NOTE: If the compound does not dissolve entirely, heat the flask using a heat gun and agitate the flask as needed to completely dissolve the compound.
2. Make phosphate buffered saline (PBS) solutions at various pH levels. The analogs were characterized in PBS buffers ranging in pH from approximately 4 to 15.
   1. Using a 1 L volumetric cylinder, create 1 L of PBS stock solution by diluting 100 mL of PBS (x100) in 900 mL of deionized water.
   2. Transfer 50 mL of the prepared PBS stock solution (step 3.2.1) to a 100 mL beaker and add a magnetic stir bar. Then using a calibrated pH meter to monitor changes in pH, titrate the PBS buffer with either aqueous 1.0 M NaOH (to obtain buffers with pH > 7.0) or 1.0 M HCl (to obtain buffers with pH < 7.0).
      NOTE: To obtain data that results in a well-defined titration curve, we recommend generating pH buffers in increments of 0.1 pH units within ± 0.5 of the anticipated point of inflection and increments of 0.5 outside of the anticipated point of inflection.
3. Acquire molar absorptivity spectra for analogs 16 - 25 in PBS (pH 7.0) and 1.0 M NaOH (pH 14.0) solutions.
   1. Prepare a "blank" using a clean and dry quartz cuvette then add 2.0 mL of either PBS stock solution (pH 7.0) or aqueous 1.0 M NaOH to the cuvette using a 100 - 1000 µL micropipette.
      NOTE: It is important to the integrity of the blank solution data acquisition process to ensure that there are no air bubbles in the cuvette solution and to thoroughly wipe the sides of the cuvette with a Kim wipe to prevent light scattering resulting from dust or debris on the outside of the cuvette. If bubbles persist, gently and repeatedly tap the cuvette on a paper towel laid on a hard surface.
   2. Using a UV/Vis spectrophotometer, blank the selected solution for a 200 - 800 nm range.
   3. Into a second clean and dry quartz cuvette add 2.00 mL of either PBS (pH 7.0) or 1.0 M NaOH (pH 14) followed by 10 µL of the dipyrrinone analog (16 - 25) stock solution (see step 3.1) with a 5-50 µL micropipette. Place a cap on the cuvette and shake well in addition to inverting the cuvette.
      NOTE: It is important to the integrity of the sample solution data acquisition process to ensure that there are no air bubbles in the cuvette solution and to thoroughly wipe the sides of the cuvette with a Kim wipe to prevent light scattering resulting from dust or debris on the outside of the cuvette. If bubbles persist, gently and repeatedly tap the cuvette on a paper towel laid on a hard surface.
   4. Using the UV/Vis spectrophotometer, acquire an absorption spectrum for the dipyrrinone analog solution for a 200 - 800 nm range.
   5. To the same cuvette, add an additional 10 µL of the dipyrrinone analog stock solution and repeat steps 3.3.3 and 3.3.4.
   6. Repeat step 3.3.5 until a total of 50 µL of dipyrrinone analog stock solution has been added to the cuvette in order to acquire at least five excitation wavelength data points. Repeat steps 3.3.1 - 3.3.6 until all stock solutions of 16 - 25 have been obtained in both PBS (pH 7.0) and 1.0 M NaOH.
4. Obtain molar absorptivity values for 16 - 25 in PBS (pH 7.0) and 1.0 M NaOH using best fit linear regression analysis.
   1. Using a graphing program such as GraphPad Prism 7, plot the measured absorbance (y-axis) against the dipyrrinone analog concentration (x-axis). Create a best-fit linear regression analysis for the five plotted points. A linear relationship should be observed and statistical analysis should show an R² value ≥ 0.98.
   2. Repeat step 3.4.1 for analogs 16 - 25 in PBS (pH 7.0) and 1.0 M NaOH.
   3. Calculate the molar absorptivity for 16 - 25 in PBS (pH 7.0) and 1.0 M NaOH using the extrapolated slope value from the best fit linear curve.
5. Determine the pK_a values of 16 - 25 Studies Using UV/Vis Spectrophotometry
   1. Into a clean and dry quartz cuvette, transfer 1,900 µL of the PBS buffer at the selected pH level (prepared in Step 3.2) using a 100 - 1000 µL micropipette.
      ​NOTE: We have noticed upon storage that a white precipitate can form in some of the buffers. To ensure that the buffer is completely homogenous and if any precipitate is visible, use gravity filtration to remove any precipitate immediately prior to use. See the note after step 3.3.1.
   2. Using the UV/Vis spectrophotometer, blank the selected PBS buffer solution for a 200 - 800 nm range.
   3. Into a second clean and dry quartz cuvette, transfer 1,900 µL of the selected PBS buffer then add 100 µL of the selected analog stock solution using a 5-50 µL micropipette. Place a cap on the cuvette and shake well in addition to inverting the cuvette.
      ​NOTE: See the previous note after step 3.3.3.
   4. Using the UV/Vis spectrophotometer, acquire the absorption spectrum for the dipyrrinone analog for a range of 200 - 800 nm.
   5. Repeat steps 3.5.1 - 3.5.4 for 16 - 25 in each of the PBS buffers generated in step 3.2.
6. Determine the pK_a values for 16 - 25 using a best fit sigmoidal curve fitting function.
   1. Using a graphing program, graph the measured absorbance vs. wavelength (nm) for 16 - 25 at the various pH levels.
   2. Choose a wavelength between 380-415 nm where at lower pH levels (< 7.0) absorbance is small (0-0.1 units) and at greater pH (> 12.0) absorbance is considerably larger (0.8-1.0 units). Plot the absorbance at the chosen wavelength vs. the pH.
   3. Using a sigmoidal curve function, generate a best fit curve for each of the analogs 16 - 25. Report the extrapolated pH at half-height of the curve. This is the reported pK_a value.

4. Quantum Yield Acquisition and Fluorescence Studies

1. Create fluorescence study stock solutions for dipyrrinone analogs 16 - 18 and 20 - 25.
   1. Using a dipyrrinone analog stock solution created in step 3.1, perform a dilution of the stock solution by adding 10 µL of the stock solution to a 1 mL volumetric flask using a 2 - 20 µL micropipette, then add PBS (pH 7.0) buffer to the 1 mL mark. Place a cap on the volumetric flask and mix well by inverting and shaking the flask. This diluted stock solution will be used to generate the fluorescence spectra and will be referred to as the fluorescence stock solution.
   2. Repeat step 4.1.1 for analogs 16 - 18 and 20 - 25.
2. Acquire fluorescence emission spectra at varying concentrations, for analogs 16 - 18 and 20 - 25. For all analogs other than 18, acquire five spectra for each analog in solutions of pH 7 and 14 at concentrations of: 19.96, 39.84, 59.64, 79.37, and 99.01 nM. For analog 18, acquire five spectra in a solution of pH 7 at concentrations of: 49.75, 99.01, 147.8, 196.1, and 243.9 nM. In a solution of pH 14, acquire five spectra for analog 18 at concentrations of: 99.01, 196.1, 291.3, 384.6, 476.2 nM.
   1. Into a transparent four-sided quartz cuvette, add 3.00 mL of PBS (pH 7.0) or 1.0 NaOH using a 100 -1,000 µL micropipette in three 1,000 µL increments.
      ​NOTE: See note after step 3.3.1.
   2. Using the fluorometer and the fluorometer software program FluorEssence, acquire an emission spectrum for the selected solution and label this as the solution "blank".
   3. To the same cuvette, add 6 µL of fluorescence stock solution for the selected dipyrrinone analog (Part 4.1) using a 0.5-10 µL micropipette. Place the cap on the cuvette and mix well by inverting and gently shaking the cuvette.
      NOTE: See note after step 3.3.3.
   4. Using the fluorometer, acquire an emission spectrum for the selected compound solution using λ​_max abs as the excitation wavelength. Excitation intensity was measured over a 200 nm range starting at 15 nm beyond the excitation wavelength (typically a 200 nm range is required for the fluorescence intensity to return to baseline).
   5. Repeat steps 4.2.3 - 4.2.4 until a total of 30 µL of fluorescence stock solution has been added to the cuvette.
   6. Repeat steps 4.2.1 - 4.2.5 for analogs 16 - 25 in PBS (pH 7.0) and 1.0 M NaOH.
      NOTE: The cuvette concentrations were changed for analog 18 and data was acquired using: 2.0 mL of PBS with five consecutive 10 µL increments of fluorescence stock solution for a neutral (protonated 18) solution and 2.0 mL of 1.0 M NaOH with five 20 µL increments of added compound stock solution for a basic (deprotonated 18) solution.
3. Determine the quantum yield using the method of Williams, A. T. et al.¹⁷
   1. Using a spreadsheet software program (i.e., Microsoft Excel), import the data (emission intensity data points) for the emission spectra for a single dipyrrinone analog (taken in either PBS [pH 7.0] or 1.0 M NaOH) at the various concentration levels.
   2. Import the data points from the emission spectra for the "blank" solution (steps 4.2.1 - 4.2.2) and subtract the "blank" emission intensity data points from the emission intensity data points, at the corresponding wavelengths, acquired at various concentration levels.
   3. Transfer the "blank" corrected emission intensity data points into a graphing program, such as GraphPad Prism 7, and plot emission vs. wavelength. Calculate the area under the curve for each of the curves obtained at the various concentration levels of dipyrrinone analog.
   4. Following the technique outlined by Williams, A. T. et al, calculate an extrapolated absorbance value for each of the varying concentration levels of dipyrrinone analog. This is accomplished by multiplying the calculated molar absorptivity value (from best-fit linear regression analysis, see step 3.4) by each concentration of dipyrrinone analog used in steps 4.2.3-4.2.5.
   5. Using a graphing program such as GraphPad Prism 7, create a plot of the analog's extrapolated absorbance (x-axis) against the calculated area under each concentration curve (step 4.3.4) for the emission wavelength corresponding to the greatest emission value. A linear relationship with a r² ≥ 0.96 should be observed.
   6. Perform steps analogous to 3.1-3.4 and 4.1-4.3.5 for quinine in 0.5 M H₂SO₄ (Φ_F = 0.55)¹⁸ and anthracene in ethanol (Φ_F = 0.27)^{18, 19} to obtain data for standards.
   7. Obtain the quantum yield values for 16 - 25 in PBS (pH 7.0) and 1.0 M NaOH by using the extrapolated slopes obtained from steps 4.3.5 and 4.3.6 in the following equation:
      Φx =Φ_st(Grad_x/Grad_st)(η​²_x/η²_st)
      where Φ_st represents the quantum yield of the standard, Φ_x represents the quantum yield of the unknown, Grad is the slope of the best linear fit, and η is the refractive index of the solvent used (the refractive index ratio was calculated using η = 1.36 for ethanol and η = 1.35 for 0.5 M H₂SO₄).
   8. Report the quantum yields for 16 - 18 and 20 - 25 in PBS (pH 7.0) and 1.0 M NaOH as an average of the Φ_x obtained for quinine and anthracene.

Wyniki

The Claisen-Schmidt condensation reaction provided access to dipyrrinone analogs (16-25, Figure 4) using the one-pot procedure described in the protocol section (see step 1). Analogs 16-25 were all generated by condensing pyrrolinone 9, bromoisoindolone 10, or isoindolone 11 with 1H-imidazole-2-carboxaldehyde (12), 1H-imidazole-5-carboxalde...

Dyskusje

The Claisen-Schmidt condensation approach provides a fairly robust means of generating pyrazole, imidazole, and isoindolone dipyrrinone fluorophores through a relatively operationally simplistic protocol. While the synthesis of the fluorescent dipyrrinone analogs was the focus of this study, it should be noted that similar conditions can be applied to access other bicyclic methine-linked ring systems such as dipyrrinones^23,24,25...

Materiały

Name	Company	Catalog Number	Comments
3-ethyl-4-methyl-3-pyrrolin-2-one	Combi-Blocks	[766-36-9]	Yellow solid reagent
isoindolin-1-one	ArkPharm	[480-91-1]	Off-white solid reagent
5-bromoisoindolin-1-one	Combi-Blocks	[552330-86-6]	Pink solid reagent
2-formylimidazole	Combi-Blocks	[10111-08-7 ]	Off-white solid reagent
Imidazole-4-carbaldehyde	ArkPharm	[3034-50-2]	Solid reagent
1-H-pyrazole-4-carbaldehyde	Oakwood Chemicals	[35344-95-7]	Solid reagent
1-H-pyrazole-5-carbaldehyde	Matrix Scientific	[3920-50-1]	Solid reagent
Solid KOH Pellets	BeanTown Chemicals	[1310-58-3]	White solid pellets
Siliflash Silica Gel	Scilicycle	R12030B	Fine white powder
Phosphate Buffered Saline (PBS) (x10)	Growcells	MRGF-6235	Colorless translucent liquid
Beckman Coulter DU-800 UV/Vis Spectrophotometer and Software	Beckman Coulter	N/A	Spectroscopy Instrument and Software
Fluoromax-4 Spectrofluorometer	Horiba Scientific	N/A	Spectroscopy Instrument
FluorEssence Fluoremetry Software V3.5	Horiba Scientific	N/A	Spectroscopy Software
Finnpipette II Micropipette (sizes: 100-1,000, 20-200, and 0.5-10 µL)	Fischerbrand	N/A	Equipment
Wilmad-LabGlass Rotary Evaporator (Model: WG-EV311-V-PLUS)	SP Scienceware	N/A	Equipment
DuoSeal Vacuum Pump (Model Number: 1405)	Welch	N/A	Equipment
GraphPad Prism 4	GraphPad	N/A	Data Analysis Software
SympHony pH Meter (Model: Sb70P)	VWR	N/A	Equipment