JoVE Logo

Zaloguj się

Aby wyświetlić tę treść, wymagana jest subskrypcja JoVE. Zaloguj się lub rozpocznij bezpłatny okres próbny.

W tym Artykule

  • Podsumowanie
  • Streszczenie
  • Protokół
  • Ujawnienia
  • Odniesienia
  • Przedruki i uprawnienia

Podsumowanie

In this video, we demonstrate a method for electrophoretic separation of proteins using poly-acrylimide gel electrophoresis (PAGE).

Streszczenie

Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to investigate subunit compositions, and to verify homogeneity of protein samples. It can also serve to purify proteins for use in further applications. In polyacrylamide gel electrophoresis, proteins migrate in response to an electrical field through pores in a polyacrylamide gel matrix; pore size decreases with increasing acrylamide concentration. The combination of pore size and protein charge, size, and shape determines the migration rate of the protein. In this unit, the standard Laemmli method is described for discontinuous gel electrophoresis under denaturing conditions, i.e., in the presence of sodium dodecyl sulfate (SDS).

Protokół

The complete text protocol for this experimental approach is available in Current Protocols in Molecular Biology.

Ujawnienia

The authors have nothing to disclose.

Odniesienia

Przedruki i uprawnienia

Zapytaj o uprawnienia na użycie tekstu lub obrazów z tego artykułu JoVE

Zapytaj o uprawnienia

Przeglądaj więcej artyków

Electrophoretic SeparationProteinsProtein SeparationElectrophoresisComplex MixturesSubunit CompositionsProtein SamplesPurification Of ProteinsPolyacrylamide Gel ElectrophoresisElectrical FieldPore SizeAcrylamide ConcentrationProtein ChargeProtein SizeProtein ShapeMigration RateLaemmli MethodDiscontinuous Gel ElectrophoresisDenaturing ConditionsSodium Dodecyl Sulfate SDS

This article has been published

Video Coming Soon

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone