1. Transferring Objects into the Glovebox

1. Make sure both doors are closed (this should be the default position). If the chamber is under vacuum, fill it with nitrogen by turning the valve to fill.
2. Open the outer door, and transfer the object in the chamber. Slide the object as deep as possible, which makes it easier to reach it from inside the glovebox. Close the door isolating the antechamber from the ambient environment. DO NOT over tighten the door, go only a quarter-turn further.
3. Once the doors are closed, turn the valve to evacuate the chamber, and wait until the pressure is at an acceptable vacuum level. Then fill the chamber with nitrogen, by turning the valve to refill. Repeat this cycle at least 3 times.
4. Once the purging and evacuation cycles are complete, fill the chamber with nitrogen, and then open the inner door to the glovebox. Remove the object from the antechamber and place in a safe location in the glove box. Promptly close the door to the antechamber.
5. With the doors closed, turn the valve on the antechamber to vacuum, and leave it there. This is the default position of the antechamber when not in use.

2. Transferring Objects Out of the Glovebox

1. Make sure the antechamber is filled with nitrogen. If you are unsure about the state of the chamber, evacuate and refill the chamber with nitrogen at least 3 times.
2. Open the inner door, and transfer the object into the chamber. If the object includes chemicals, make sure it is properly sealed. Then close the inner door to the antechamber.
3. Open the outer door and remove the object(s).
4. Close the outer door and turn the valve to evacuate. The default condition for the antechamber is to be under vacuum.

3. Chemicals and Apparatus

1. Make sure all of the apparatuses that you transfer into the glovebox are dry.
2. Any chemical that is taken in the glove box must be dry and deoxygenated. It is best to use DriSolv solvents. If these are not available, it would be preferred to use solvent bottles which have not been opened outside of the glovebox.
3. Take only the required amount of chemicals into the glovebox, full reagent bottles MUST NOT be taken in (unless the reagent has to be permanently stored in the box).
4. Use 20-mL vials to transfer solids and non-volatile liquids. Wrap the top of the vials with lab wipes and use a rubber band to hold the wipe in place. This allows the air above the solid/liquid to be evacuated.
5. While transferring volatile liquids, use Teflon stoppered flasks.
6. Be extremely careful while using needles and blades in the glove box. The gloves are extremely delicate, and any puncture can compromise the atmosphere in the box.
7. Once you have finished your work, take out all the apparatuses promptly and clean the area.
8. Store any chemicals that have been synthesized in an appropriate location within the box and label it appropriately.
9. Let the person in charge of the box know if any of the glovebox-dedicated consumables are running low.

4. Solvents

1. While taking in solvents, make sure to use the Teflon-stoppered round-bottom flasks, and only use dry deoxygenated solvents from the solvent stills or as ordered from chemical suppliers.
2. All the solvent bottles stored in the box should be capped all times.
3. Solvent vapors can damage the gloves, and hence any solvent spills must be promptly wiped with lab wipes, and then removed since volatile solvent vapors will remain.
4. Place the solvent bottles back in the appropriate location once you have finished using them.

5. Waste Disposal

1. Dispose of the waste in properly labeled waste beakers.
2. Recap the needles after use before disposing of them.
3. If the waste container is full after you use it, take it out of the box, and dispose of it properly in the lab waste, and promptly replace the waste container in the glovebox.
4. Clean up any spills on the glove box floor and dispose waste in the misc. waste.
5. The liquid waste bottle MUST be capped at all times.

6. General Tips

1. Do not insert/remove your hands from the gloves quickly. Doing so quickly will cause a sudden pressure change in the box, which can lead to ambient air from the external environment accessing the glovebox through any micro pores on the gloves.
2. Be extremely careful while using needles and blades in the glove box. The gloves are extremely delicate, and any puncture can compromise the atmosphere in the box.
3. Make sure the chambers are always left under vacuum after use.
4. Shut off the blower using the main screen when handling amines, phosphines, or any other volatiles, since they can damage the catalysts.
5. Keep a logbook of all glovebox activity including maintenance performed on the glovebox.
6. Conduct maintenance (vacuum pump oil change, regeneration of purification system) on a regular schedule.