Cryosectioning of the Dorsal Root Ganglion (DRG)

Begin the cryosectioning by holding the optimal cutting temperature DRG section at the bottom, using a small paint brush, pre-chilled at 20 degrees Celsius. Gently touch the bottom of the section using the end of a room temperature tweezer so that it sticks to the platform surface. Next, slowly place a charged glass slide over the section.

As soon as the section sticks to the slide, gently pull the slide backward. Follow the same process to get more DRG sections onto the slide without overlapping the sections. The proper placement of the DRG sections on the glass slides is seen where the tissues are not overlapping the DRG section and the OCT of other DRG sections.

The confocal fluorescence microscopy image demonstrated the quality of the DRG sections obtained with this protocol. With this technique, the immunohistochemistry studies of DRG can be performed using different antibodies.