Begin by thawing the Selleckchem Small Molecule Compound Library plates at room temperature. Open the software and click on New Protocol. On the Protocol tab, click on Sample Plate Format, followed by 384PP.
Then on Sample Plate Type, select 384PP_DMSO2. Next, choose Cell Carrier 384 Ultra PN as the Destination Plate Type. Click on Pick List and select the import options to import the spreadsheet containing the dispensing program.
Click on the Run Protocol option to verify the accuracy of the displayed information and then click on Run. Next press Start on the new window titled Run status, and then follow the steps displayed on the prompt windows. Use a nanoscale acoustic liquid dispenser and dispense 50 nanoliters per well of the Small Molecule Compound Library into a 384 well assay plate.
To begin cellular supernatant harvesting, start by configuring the deck of the lab automation workstation. Place a disposable 96 tip rack on the P1 position and place the assay plates on positions P2 and P4.Finally, place the empty, solid black 384 well plates at positions P3 and P5.Using the tips from P1, aspirate 10 microliters of the supernatant from the plate at P2 and then transfer it into the empty solid black plate on P3.Discard the tips at position P6.Repeat the transfer of supernatant from P4 to P5.
