Administration of Fu's Subcutaneous Needling (FSN) Manipulation in a Chronic Constriction Injury Rat Model

1 Begin by securing the rat2 from Fu's subcutaneous needling 3 or FSN treatment group in the rodent, restrained, 4 exposing the affected limb laterally. 5 Slowly and gradually extend the rat's hind limbs 6 until stretched tight without anesthesia. 7 Now, remove the protective sheath of the FSN needle.

8 Insert the needle tip toward the tightened muscles 9 near the gluteus maximus muscle 10 on the lower back and rear, 11 position the needle flat entering the skin at a 12 15 degree angle. 13 Push it cautiously 14 and swiftly to avoid tension in the rat, 15 ensuring full insertion of the needle 16 and burying the soft tube under the skin. 17 Begin the swaying movement by smoothly 18 and softly fanning the FSN needle tip, using the thumb 19 as the fulcrum and keeping the index, middle, 20 and ring fingers aligned in a straight line.

21 Hold the FSN needle between the middle finger 22 and thumb facing each other 23 and alternate the movement using the index 24 and ring fingers, upon completing the manipulation, 25 quickly withdraw the FSN needle. 26 The sciatic function index 27 or SFI in the chronic constriction injury, 28 FSN Group improved significantly 29 compared to the injury group alone. 30 The improvement was significant in the FSN group 31 relative to the transcutaneous electrical nerve stimulator 32 or TENS group.

33 The SFI of the sham, FSN, and TENS groups 34 did not have any significant 35 difference from each other, 36 proving the safety of the treatment groups. 37 The amplitude of CMAP 38 and the latency peaks in the injury FSN treatment group 39 improved significantly compared to the injury group alone. 40 The CMAP and latency peaks 41 of the treatment groups did not differ 42 significantly from each other.

43 The CMAP amplitudes, and latency 44 of all three groups were statistically similar.