To begin, thoroughly wash the microscope glass slide with water. Then, after dipping the slides in methanol, allow them to dry under ambient conditions on a dust-free wipe. Next, using an automatic pipette, transfer 100 microliters of the insulin spherulite solution to the well in the center area of the microscope slide.
Carefully cover the solution with a cover slip, avoiding air bubble formation. Then, using an automatic pipette, deposit the mountant along the edges of the cover slip on the slide to seal the native spherulite solution. Leave the sample under ambient conditions for the mountant to harden.
Using a polarized optical microscope with crossed polarizers, confirm if insulin spherulites have formed correctly. Scan through the sample, looking for a characteristic pattern of bright areas, known as a Maltese cross. The polarized optical microscopy revealed the presence of the Maltese cross in the spherulites.
