To manually extract the Bradford Assay data from the images of the microplate recorded using the smartphone camera, download Inkscape, a free and open source graphics editor. Open Inkscape, go to file, and click open"to select the picture of the microplate taken using the smartphone. Pick the select and transform objects S tool, which is depicted as an arrow on the top left side, and click on the picture.
A dashed line border will indicate the selection. Then select the pick colors from Image D tool, which is depicted as an eyedropper on the left side. Next, click in the center of a well.
The color on the bottom panel, fill, will change accordingly. Click on the color and a fill and stroke tab will pop up on the right side. Change the flat color dropdown menu to RGB.
Record the values shown for the blue and green channels for each well. Calculate the blue to green ratio using the recorded values.
