Preparing a Quercetin-Containing Chickpea-Based Artificial Diet for Evaluating its Effect on Helicoverpa armigera Growth

To begin, weigh the desired components of fractions A, B, and C separately in a beaker, and prepare a homogenous mixture using a spatula or magnetic stir. Boil fraction C at around 100 degrees Celsius in a microwave for five minutes. Then add it to fraction A and mix thoroughly.

Once the mixture cools down, add fraction B and pour the prepared control diet mixture into a transparent polystyrene Petri dish. To prepare quercetin containing an artificial diet, dissolve quercetin hydrate and dimethyl sulfoxide, then add the dissolved quercetin into fraction B, followed by a mixture of fractions A and C.Add an equivalent dimethyl sulfoxide to the prepared controlled diet.