This protocol provides a useful reproductive model for the studies of antigen specific CD8 T-cells. They are lymph node metastasis, which excludes the participation of bystander CD8 T-cells. We try to reveal the systemic and local dynamics of antigen specific CD8 T cells They are lymph node metastasis.

Recent accumulating evidence revealed that tumor specific CD8 T cells derived from the periphery, especially in tumor draining lymph node, but not in tumor microenvironment met the efficacy of immune checkpoint blockage therapy. Recently we identified this in one positive talks negative tumor sub specific memory T cells in tumor draining lymph nodes of both mice tumor models and human hepatocellular carcinoma patients which serve as general responders to immune checkpoint blockage. It is difficult to evaluate the precise time points of intubation because lymph nodes metastasis through other techniques is not always feasible.

This protocol has provided an approach to precisely investigate the antigen specific CD8 T-cells immune responses. The lymph node metastasis, which excludes the participation of bystander CD8 T cells. Our experimental design provides a useful model to investigate the antigen specific CD8 T-cells, the lymph node metastasis and interactions between antigen specific CD8 T cells and other immune cells or stroma cells during lymph node metastasis.

We'll try to elucidate how lymph node metastasis affects the anti-tumor immune response, especially the properties and the functions of TDR and TTSM cells. This results would affect the clinical treatment options of whether to remove or retain the MLN and show the new light on the manipulation of MLN to achieve maximum therapeutic benefits.