After preparing the paraffin sections of the kidney and Peyer's patch isolated from immunoglobulin A or IgA nephropathy model mouse, proceed to dewax and stain the desired paraffin section using periodic acid solution at room temperature for 10 minutes while avoiding exposure to light. Rinse the section with distilled water before blotting it dry. Then stain the dry section with Schiff's staining solution for 20 to 30 minutes while avoiding exposure to light.
Rinse the section with distilled water until it appears red under the microscope. Immerse the rinsed section in hematoxylin staining solution for three minutes to stain the nuclei and then rinse it under running water until the section becomes colorless. After performing routine dehydration with xylene and ethanol gradients, seal the section with neutral gum to examine it under a microscope.
Compared with the control group, the mucosal immune induced IgA nephropathy mice model has visible IgA deposition in the entire mesangial region. PAS staining of the renal tissue showed mesangial cell proliferation and stromal hyperplasia in the model group, which was reduced in the dioscin gavage group.
