To begin, place a 60-millimeter Petri dish covered with 1%agarose and PBS in an egg-laying chamber. Put a drop of liquid yeast in the center of the plate to encourage egg-laying. Next, introduce 300 gypsophila flies into the chamber.
Keep the flies at 25 degrees Celsius for three days. Replace the Petri dish in freshly dissolved yeast twice daily. On the fourth day, let the flies lay eggs for four hours.
After replacing the dish, allow the flies to lay eggs for three more hours on the fresh plate. After three hours, place the plaque with the eggs at 25 degrees Celsius for 24 hours. From this plaque, collect 50 to 100 newly-hatched larvae and place them in a plastic vial containing standard feed.
