Mouse Brain Histology to Study the Pathological Mechanisms of Ischemic Stroke Induced by Distal Middle Artery Occlusion

To begin, obtain the dissected brain of the euthanized distal MCAO mouse model after behavioral testing, and place it in a minus 20 degrees Celsius freezer for 20 minutes. Then position the brain into a one-millimeter brain matrix. And using microtone blades, slice the brain into two-millimeter-thick coronary sections.

Transfer the brain sections to a 24-well culture plate and add 2%TTC to cover the brain tissue. Incubate the plate at 37 degrees Celsius for 30 minutes. Then carefully fix the brain section in 4%Paraldehyde solution for 15 minutes.

Using a scanister, perform optical scanning of the brain sections. The histological analysis of the brain revealed the disordered arrangement of neuron cells and a significant reduction in the density of NeuN positive cells in the peri-infarct area of the distal MCA occluded mice.