1 To begin, launch the Cytoscape 3.9.1 software2 on the desktop and import the tsv file 3 containing core proteins of anus eczema. 4 Click on the style bar in the control panel 5 to optimize the color, font, and size of the network nodes. 6 For network topology analysis, 7 employ the analyze network function.
8 To obtain hub genes, use CytoHubba 9 and establish the drug component disease target network. 10 Open the Metascape website. 11 Select a file or paste a gene list into the dialog box 12 and click the submit button.
13 Then select H.sapiens in both input as species 14 and analysis as species, 15 and enable the custom analysis function. 16 In the enrichment option, 17 select GO molecular functions, GO biological processes, 18 GO cellular components, and the KEGG Pathway database. 19 Check pick selective GO clusters, 20 and click on the enrichment analysis button.
21 Upon completion of the progress bar, 22 initiate an analysis report page and click 23 to retrieve the enrichment results. 24 Open the GEO2R tool to search 25 and analyze the GEO gene chip database. 26 Open the GEO database website.
27 Then enter the keyword or GEO accession 28 and click on the search button. 29 Select the best matching result, 30 and find the reference series. 31 Now on the GEO2R tool website, 32 enter the reference series in the GEO accession box 33 and click the set button.
34 Select atopic dermatitis as the experimental group 35 and nonatopic control as the control group. 36 Click the analyze button and wait for the results to appear. 37 KEGG and GO enrichment analyses of 59 key targets 38 identified 218 pathways and over 3, 000 biological processes, 39 highlighting significant pathways in SDG and anal eczema.
40 GO analysis on biological processes, cell composition, 41 and molecular function 42 emphasized common targets in SDG and anal eczema. 43 Relevant biological functions included peptidyl-tyrosine 44 phosphorylation and regulation of cell-cell adhesion.
