Preparation and Inoculation of Trypanosoma evansi in Mouse to Evaluate Allodynia and Pain

To begin, turn on the water bath equipment and set it at 37 degrees Celsius, remove a micro centrifuge tube containing the preserved blood sample from the ultra freezer and place it in the water bath until the blood thaws. Perform serial dilution using PBS with 60%glucose to dilute the trypanosomes. Count the number of parasite cells using a neubauer chamber under a microscope with a 100 times objective lens.