Mechanical Threshold Assessment of Mice Infected with Trypanosoma evansi

Inject the experimental mice intraperitoneally with 0.1 Milliliters of diluted blood solution infected with Trypanosoma evansi. For the control group, inject 0.1 milliliters of PBS vehicle intraperitoneally into the mice. One hour later, set up the EVF apparatus for mechanical threshold assessments.

For the day zero assessment, after one hour of infection, and 30 minutes of acclimatization of the mice, evaluate the mechanical threshold for the right hind paw or visceral allodynia. After taking the measurements, return the mice to their original cages to avoid conflicts among them and provide standard lab chow and water ad libitum.