To begin, fit the columns to a clamp stand as straight as possible. Plug the bottom of each column with a stopper. Add one to two milliliters of starting buffer to the bottom of each column to prevent air bubbles during column packing.
Then open the top of the columns and pour in 10 milliliters of each slurry continuously against a metal spatula or glass rod held at the rim of the column to prevent air bubbles. Remove the stopper from the column and connect the column's bottom to one end of the tubing. Attach the other end of the tubing to the UV port of the FPLC system.
Verify that the bed support housing is pulled into the flow adapter body and slide the flow adapter body onto the top of the column. With the cam latch in position one, lower the flow adapter. Then switch the cam latch to position two.
Connect the tubing to the system and start the buffer flow for one to two minutes to eliminate air from the tubing and prevent air bubbles during packing. Afterward, switch the latch back to position one, lower the flow adapter smoothly into the buffer, avoiding air bubbles, and switch the latch back to position two. After switching the latch from position two to three, tighten both the cam base and the locking ring.
Then, start the buffer flow at 10 milliliters per minute for three minutes or until the resin is well packed.
