To begin, design DNAzyme-based nanomachines, or DNM, with a 10 to 23 core, using free software. Obtain the designed sequences from commercial sources. Then, take 0.5 milliliter microcentrifuge tubes with 200 microliters of the reaction buffer.
Add DZB-Tile and Tile-arm3 oligonucleotides to the tubes. Gently mix them and spin down the solution. Wrap the lid of the closed tube with paraffin film or use screw cap tubes.
Incubate the tube in a 500 milliliter beaker with boiling water for two minutes. Then, turn the heater off and allow the temperature to passively cool down overnight. Now, prepare the 12%native page using the given reagents.
Transfer the mixture to the gel cassette and allow it to polymerize. Mix one microliter of each sample with one microliter of four times loading dye and load them into the gel. Place the cassette into the chamber.
Fill it with TBE buffer and run the gel for 90 minutes at 82 to 100 volts. Dye the gel with ethidium bromide and visualize it using a gel documenting system.
