Zebrafish Ovary Excision for Obtaining Granulosa Cell-Free Stage I Oocytes

To begin, place a euthanized female zebrafish on a table. Dab the excess water on the fish's surface with absorbent paper. With a pair of micro-scissors, cut off the head of the fish along the posterior margin of the gill cover.

Then cut off the tail with the cloaca. Transfer the middle trunk to a 35-millimeter wide dish with 2 milliliters of L-15 medium. Now, cut the central axis of the abdomen and remove the viscera and swim bladder.

Use a pair of tweezers to detach the bilateral ovaries from the abdomen. Remove the adipose tissue, scales, and other body tissues attached to the ovary.