To begin, segregate 50 hypertensive rats into five groups, including control. Give the rats in Group 1, methionine or met group, a diet of 3%methionine for 28 days to induce H-type hypertension. For Group 2 to 4 rats, administer a combination of Huotan Jiedu Tongluo Decoction and enalapril maleate in addition to the methionine diet for 28 days.
Next, create a restraining device by placing a cylindrical restraint mesh into a thermal tube and putting the thermal tube into a canvas cover. After 28 days of treatment, place a rat in the mesh. Next place the signal cable of a noninvasive sphygmomanometer under the cover.
Position the rat's tail in the gap in the cover and secure it on the stabilizing form pad. Transfer the rat to a quiet measurement site 20 to 30 minutes before blood pressure measurement. Now connect the pressurized sensor's air hose connection, signal connection, and holding tube.
Place the pressurization sensor at the tip of the rat's tail. A pulse wave should appear after the rat tail is inserted into the sensor. Press Start/Stop to initiate or stop the measurement.
When the blood pressure test is completed, the result menu will pop up automatically. Check the average value of the measurement, standard deviation, standard, and coefficient of variation in the results menu. After euthanizing the rat, dissect it with a pair of surgical scissors and tooth forceps from the perineum to the neck.
Turn over the thoracic and abdominal contents. Then navigate to the abdominal aortic bifurcation between the two kidneys. Collect the aorta up to a section of the aortic arch.
The systolic and diastolic blood pressures were significantly greater in the Met Group relative to the control group from one to four weeks. The blood pressures decreased after the administration of the Huotan Jiedu Tongluo Decoction. The combined effect of the decoction and/or enalapril maleate had a stronger anti-hypertensive effect than the decoction alone.
