Performing Transcranial Ultrasound Neuromodulation Experiment in Humans

After performing the skull reconstruction and simulations using the acquired MRI scans, open Brainsight and click on Open Existing Project. Select the Brainsight file created and saved during trajectory and target mapping. Click on Sessions to begin a new experimentation session.

Click on the dropdown and select New, followed by Online Session. Then, click on the target name followed by Add a Next Step to add the target to the experiment session and continue to the experimentation window. Click on Window and Tool Calibration.

Select the tools to be used and then click Recalibrate. Attach the subject tracker to the participant's head using a headband placed above the ears and eyebrows. Next, click on the registration tab in Brainsight.

Place the pointer on the first landmark, stabilizing it with both hands. Then click on Sample and go to next landmark. Click on the Validation tab to validate the participant registration.

Lightly place the pointer on various positions along the scalp and ensure all points are less than three millimeters. Then secure the participant's head with the chin rest and stabilizer placed on the back of the head to prevent movement. Ensure they are seated comfortably in the chair for the duration of the experiment.

Click on Perform. Use the mechanical arm to position the transducer over the target of interest along the selected trajectory.