MN-anti-miR10b Administration in Murine Glioblastoma Models for Bioluminescence and Fluorescence Imaging

To begin, weigh the mouse and calculate the volume of magnetic nanoparticle anti-microRNA-10b to administer for a dosage of 20 milligrams iron per kilogram in glioblastoma multiforme. Without introducing bubbles, prepare a 28-gauge syringe with the dose of magnetic nanoparticle anti-microRNA-10b. Sterilize the mouse tail with a 70%isopropanol wipe.

After drying, rotate the tail to position the lateral caudal veins at the top of the tail. Then, insert the needle into the tail, and pull back the plunger to establish a vacuum. Continue inserting the needle until the blood enters the syringe.

Slowly inject magnetic nanoparticle solution, and retract the needle after complete delivery. Apply pressure to the injection site with gauze until the bleeding stops.