To begin extract the DNA from formal and fixed paraffin embedded tissue samples. Then centrifuge the primer dry powder at 5, 400 to 8, 400 G for two to three minutes, and add the appropriate volume of double distilled water along the wall of the primer tube. Add five microliters each of the forward and the reverse primer to 50 microliters of wild type primer stock, and 40 microliters of double distilled water.
After mixing two to three times, vortex the tube and centrifuge it briefly. For the polymerase chain reaction or PCR procedure, add the required reagents to each reaction tube. Vortex to mix and centrifuge briefly.
Place the PCR tube in the PCR instrument for amplification. Set the thermal cycle program and run it. Then perform gel electrophoresis on the PCR product using 2%Agarose to check whether the target fragment is amplified.
To purify the PCR products, briefly centrifuge the purificase one and purificase two that have been brought to room temperature and prepare the purification reaction mixture. Place the PCR tube in the PCR instrument and run the machine with the preset parameters.
