Isolation of Rat Intestinal Tubes to Study Gastrointestinal Dynamics

To begin, prepare a physiological salt solution, or PSS. Saturate the solution by bubbling with a mixed gas of 95%oxygen and 5%carbon dioxide. Maintain the pH values of the solution between 7.38 and 7.42, with two millimolar sodium hydroxide.

Pre-cool one-third of the PSS to four degrees Celsius. Then, pre-warm the remaining solution to 37 degrees Celsius for subsequent experiments. Now gather Petri dishes filled with four degrees Celsius PSS, surgical tweezers, and scissors.

Quickly place the stomach and intestine tubes in a Petri dish filled with four degrees Celsius PSS saturated with 95%oxygen and 5%carbon dioxide. Locate the duodenum in the pylorus of the stomach. Using tweezers, delicately lift the adjacent tissue and carefully trim it away from the intestine's edge with scissors.

Divide the intestine into one to two centimeter segments.