To begin, turn on the in vitro tissue perfusion system. Adjust the instrument's bath temperature to 37 degrees Celsius. Then, place the 37 degrees Celsius physiological salt solution, or PSS, into the bath.
Prepare a 15 centimeter surgical suture and soak it in four degrees Celsius PSS saturated with 95%oxygen and 5%carbon dioxide. Secure one end of the intestinal canal with the suture and use a steel needle hook to secure the other end. Next, install the intestinal tube.
Mount the segment with the steel needle hook at the bottom of the bath, and attach the other end of the surgical line to the transducer. Turn on the gas switch to allow bubbles to emerge in the bath. Now, open the data acquisition software and click on Start to ensure the corresponding path signal is being recorded.
Rotate the spiral axis of the bath counterclockwise to relax the intestinal tubes to their natural state. Click on Setup, Zero All Inputs to ensure that the software sets the intestinal tubes'initial tension to zero grams. Then, rotate the spiral axis of the bath clockwise to pull the tension value to one gram and stabilize it in the 37 degrees Celsius PSS for 30 minutes.
Observe the rhythmic spontaneous contraction waves in the software as this indicates a sufficient response. Next, add the test drug to the bath to study its effect on intestinal tube function. Afterward, stop the data acquisition software and perform data analysis using the software.
To edit the data board and select the analysis parameters, click on Window, Data Pad, and choose the average tension for the channel. Select the contraction curve before administration and click add to data pad. Then, select the contraction curve after administration and click add to data pad.
The average tension value before and after administration will appear on the data pad in turn. Then click on Window, Data Pad to copy data to other statistical analysis software for further analysis. Analyze other parameters such as average amplitude, average frequency, and integral area under the curve.
Replace the average tension with the respective parameter. Afterward, select the curve. Click on Edit, Copy LabChart Data to copy the data to drawing software for visualization.
The study examined the effects of various agents on the tension of isolated intestinal tube rings. Acetylcholine significantly enhanced tension in these tubes. Conversely, atropine inhibited the tension caused by acetylcholine.
Similarly, barium chloride also enhanced tension. On the other hand, both epinephrine and nifedipine inhibited tension in the isolated intestinal tube rings.
