Preparing Peanut Worm Homogenate and Gel for Fibrinogen-PAGE to Identify Fibrinogenolytic Agents

To begin, weigh 50 grams of peanut worms and add them into the homogenizer, along with 150 milliliters of saline. Homogenize at 24, 000 RPM for 60 seconds. Centrifuge the homogenate and collect the supernatant for further processes.

For fibrinogen polyacrylamide gel preparation, add 0.01 grams of fibrinogen and other reagents into a 50 milliliter glass beaker. Warm the beaker at 37 degrees Celsius for 30 minutes. After adding acrylamide, pour the separating gel mixture into a 10 well gel mold.

Then add two milliliters of double distilled water to the gel mold and incubate it for 30 minutes. Invert the mold to remove the water. Next, prepare the loading gel similarly, and pour it onto the same mold as earlier.

Finally, insert the comb for well formation.