Preparation of Mice and Carrageenan Solution for Creating a Model of Inflammatory Pain

Begin by using ICR female mice weighing 20 to 25 grams for the experiments. Allow the animals to acclimate to the experimental room environment for three days before starting the experiments. To prepare carrageenan solution, weigh 0.1 grams of carrageenan, add 10 milliliters of sterilized normal saline to a tube, add 0.1 grams of carrageenan to the tube, and vortex the tube for 30 seconds.

Then collect the 1%sterilized carrageenan solution.