iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

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58.3K Views

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10:45 min

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April 30th, 2011

DOI :

10.3791/2638-v

April 30th, 2011

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Julian Konig¹, Kathi Zarnack², Gregor Rot³, Tomaz Curk³, Melis Kayikci¹, Blaz Zupan³, Daniel J. Turner⁴, Nicholas M. Luscombe², Jernej Ule¹

¹Laboratory of Molecular Biology, Medical Research Council - MRC, ²European Bioinformatics Institute, EMBL Heidelberg, ³Computer and Information Science, University of Ljubljana, ⁴Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute

Podsumowanie

Rozdziały w tym wideo

0:05

Title

1:19

UV Cross-linking of Tissue Culture Cells

2:10

Bead Preparation

2:53

Cell Lysis and Partial RNA Digestion

3:47

Immunoprecipitation

4:26

RNA 5' End Labelling

5:08

SDS-PAGE and Membrane Transfer

6:27

Gel Purification of cDNA

8:44

Representative Results

10:00

Conclusion

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