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Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade

14.5K Views

11:55 min

March 14th, 2011

DOI :

10.3791/2673-v

March 14th, 2011


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Alloantigen specific Anergy

Rozdziały w tym wideo

0:05

Title

2:00

Preparation of PBMC and Bulk Alloanergizing Cultures

3:57

Measuring the Efficacy of Co-Stimulatory Blockade and Alloanergizing by Mixed Lymphocyte Reaction

6:48

Measuring the Specificity of Alloanergizing

9:05

Measuring Proliferation in the Primary and Secondary MLRs by Thymidine Incorporation

9:43

Results: Co-Stimulatory Blockade Reduces Proliferation in Primary MLR, and Alloanergizing PBMC Exhibit Decreased Proliferation in the Secondary MLR

10:34

Conclusion

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