Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants

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22.8K Views

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09:47 min

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March 11th, 2013

DOI :

10.3791/50333-v

March 11th, 2013

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Mauro W. Zappaterra¹, Anthony S. LaMantia², Christopher A. Walsh³^,⁴, Maria K. Lehtinen⁵

¹Department of Physical Medicine and Rehabilitation, VA Greater Los Angeles Healthcare System, ²Department of Pharmacology and Physiology, Institute for Neuroscience, The George Washington University School of Medicine and Health Sciences, ³Division of Genetics, Department of Medicine, Boston Children's Hospital, ⁴Howard Hughes Medical Institute, Boston Children's Hospital, ⁵Department of Pathology, Boston Children's Hospital, Harvard Medical School

Podsumowanie

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Cerebrospinal Fluid

CSF

Rodent Embryos

Cerebral Cortical Explants

Neural Progenitor Cells

Brain Development

CSF Isolation

CSF Proteome

Cortical Explant Culture

Rozdziały w tym wideo

0:05

Title

1:24

Micro-capillary Pipette and Embryo Preparations

2:47

Ventricular CSF Collection

5:01

Cortical Explant Dissection

6:51

Transferring and Culturing of Cortical Explant in CSF

8:30

CSF Collection Characteristics

9:20

Conclusion

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