Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

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12.3K Views

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12:44 min

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November 11th, 2014

DOI :

10.3791/52104-v

November 11th, 2014

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Shan Zong*¹, Shuyun Deng*¹, Kenian Chen¹, Jia Qian Wu¹

¹The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center, The University of Texas Graduate School of Biomedical Sciences at Houston

Podsumowanie

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RNA sequencing

EML Cells

Hematopoietic Stem Cells

Self renewal

Differentiation

Transcription Factors

Rozdziały w tym wideo

0:05

Title

2:09

Preparation of Baby Hamster Kidney (BHK) Cell Culture Medium for Stem Cell Factor Collection

3:25

EML Cell Culture and Depletion of Lineage Positive Cells

4:52

Separation of Lin–CD34⁺ and Lin–CD34^– Cells

7:15

RNA Preparation and Library Construction for High-throughput Sequencing

9:54

Results: RNA-Seq Identifies Transcription Factors that Regulate EML Self-renewal and Differentiation

11:38

Conclusion

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