Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

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12.7K Views

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08:40 min

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April 8th, 2016

DOI :

10.3791/53837-v

April 8th, 2016

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Tyler M. Bauman¹^,², Emily A. Ricke², Sally A. Drew³, Wei Huang³^,⁴, William A. Ricke²^,⁴

¹Division of Urologic Surgery, Washington University in St. Louis School of Medicine, ²Department of Urology, University of Wisconsin School of Medicine and Public Health, ³Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, ⁴O’Brien Urology Research Center, University of Wisconsin School of Medicine and Public Health

Podsumowanie

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Keywords Multispectral Imaging

Protein Expression

Co localization

Immunohistochemical Staining

Rozdziały w tym wideo

0:05

Title

0:37

Automated Image Acquisition and Analysis

1:43

Tissue Segmentation

3:48

Cell Segementation

5:16

Scoring and IHC and Co-localization

5:50

Applying the Algorithm and Batch Analysis

7:08

Results: Successful Cell and Tissue Segmentation

8:18

Conclusion

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