Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

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14.0K Views

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11:10 min

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June 29th, 2016

DOI :

10.3791/54139-v

June 29th, 2016

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Jens O. Watzlawik¹^,²^,³, Robert J. Kahoud¹^,³^,⁵, Bharath Wootla¹^,²^,³, Meghan M. Painter¹^,²^,³, Arthur E. Warrington¹^,²^,³, William A. Carey³^,⁴, Moses Rodriguez¹^,²^,³

¹Department of Neurology, Mayo Clinic, ²Mayo Clinic Center for Multiple Sclerosis and Autoimmune Neurology, Mayo Clinic, ³Center for Regenerative Medicine, Neuroregeneration, Mayo Clinic, ⁴Division of Neonatal Medicine, Mayo Clinic, ⁵Department of Pediatric and Adolescent Medicine, Mayo Clinic

Podsumowanie

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Keywords Antibody Binding

Antigen Identification

Therapeutic Potential

IgM Isotype

Biomarkers

Immunotherapeutic Agents

Cancer Research

Central Nervous System

Brain Tissue

Lysate

Immunoprecipitation

Rozdziały w tym wideo

0:05

Title

1:09

General CNS Tissue Preparation

2:42

Protein L Agarose Bead Preparation

8:44

Results: Antibody Binding Specificity for Kappa Light Chain-containing Human Antibodies

9:54

Conclusion

3:43

Antibody-Antigen-Agarose L Complex Formation and Antigen Detection in Western Blots

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